Cell Lysis CD:

The first step in nucleic acid analysis is extraction of nucleic acids from a sample. Then, other remaining materials such as cell membrane pieces and debris must be removed from the lysate leaving the nucleic acids behind. Mechanical cell disruption is the most effective at breaking down cells that have thick cell membranes (Gram-positive) and successfully extracting intact DNA, with the added benefit of leaving behind no residual substances. The Madou BioMEMS group has implemented a mechanical cell disruption and DNA clarification CD platform. This platform utilizes small lysis beads (100 μm in diameter) with magnetic disks placed inside each lysis chamber that oscillate while spinning by interaction with stationary magnets on a static CD platform. This oscillation causes shear forces, which result in lysis. Once lysed, solids are concentrated by centrifugation, and the supernatant (containing DNA) is extracted using a unique siphon valve. The platform is capable of efficiently lysing cells with thick walls and membranes in 5 mins while preserving the extracted DNA.