OBJECTIVE:
Obtain employment in the field of biomedical engineering with an emphasis on diagnostic and/or monitoring devices. The application of miniaturization technologies to the fields of biology and medicine greatly appeals to me, and so I would prefer to work on projects involving the micro-domain. My career goal is to be at the head of biomedical R&D projects in industry, as either part of a start-up or established company. I would eventually like to incorporate part-time community-college teaching into my career as well.

PROFILE
I am a self-motivated, hard-worker who thrives in a challenging environment either individually or as part of a team. I am a persistent and creative problem-solver, and always looking to branch out from my comfort zone to learn something new and gain additional skills. My communication and management skills are excellent, my refined leadership skills continue to develop, and my entrepreneurial spirit is strong. I am driven by curiosity while hard at work, but to relax I enjoy music (playing and listening), backpacking, and good food and wine.

EDUCATION
2006-Present, Ph.D., Biomedical Engineering
(Advancement to Candidacy: 8/28/07, Expected Graduation: 12/2009)
Advisor: Dr. Marc Madou
University of California, Irvine

2006, M.S., Biomedical Engineering
University of California, Irvine

2005, B.S., Electrical Engineering w/Biomedical Engineering Option
University of California, Los Angeles

2000, High School Diploma
Las Lomas High School — Walnut Creek, CA

SKILLS

SOFTWARE/PROGRAMMING
Microsoft Office                    SolidWorks                    LabVIEW
Windows and Mac OS X      Adobe Illustrator            AutoCAD
C++                                      L-Edit                             Matlab

MANUFACTURING/MICROFABRICATION
CNC Machining (2D & 2.5D)          Conventional Machining          Laser Machining
Thermal Bonding                            Adhesives Bonding                 Solvent Bonding
E-Beam Deposition                        Photolithography                     Soft-Lithography
Electrodeposition/polymerization              Profilometry                             RIE/DRIE

WET LAB/BIOLOGY
DNA/RNA handing                              PCR/other amplification assays          Blood handling
Fluorescence/absorbance assays      Gel-electrophoresis                             Well-plate format

BIOLOGY COURSEWORK
Molecular Biology          Cells, Tissues, & Organs          Biochemistry
Tissue Engineering        Quantitative Physiology           Clinical Medicine

ENGINEERING COURSEWORK
BioMEMS                              MEMS Fabrication                         Fluid Mechanics
Micro-Bio Implants                Fluorescence Spectroscopy          Circuit Design
Biomedical Microdevices      Electromagnetics and Waves        Thermodynamics

RESEARCH & DEVELOPMENT EXPERIENCE

2006-CURRENT : RESEARCH ON MICROFLUIDIC PLATFORMS FOR NUCLEIC ACID DIAGNOSTICS
UC Irvine, Dr. Marc Madou                    www.biomems.net
• Research towards doctorate degree involving construction of centrifugal microfluidic sample lysis, PCR, & DNA microarray hybridization platforms to integrate into a CD-based, sample-to-answer system for rapid, automated infectious disease diagnostics as part of a Genome Québec initiative
• Also developing a CD-based microfluidic system for Anthrax detection that combines cell lysis with real-time PCR as part of a DARPA initiative
• Using my microfabrication and manufacturing skills to manufacture disposable microfluidic devices in and out of the UC Irvine cleanroom facilities
• Fabricating and automating CD platform support hardware, including fluorescence detection systems and feedback control systems for thermocycling
• Research work has also included handling and performing relevant biological assays, such as running qPCR reactions and handling of blood/cells
• Additional duties include lab management, webmaster (2006-2008), and numerous grant writing efforts (NIH, NSF, and others)

2006-CURRENT : CONTINUOUS GLUCOSE MONITORING RESEARCH & DEVELOPMENT
iGlyko, Inc.                              www.iglyko.com
• Working part-time on the development of an implantable, intravenous, fiber-optic glucose sensor for tight glycemic control using genetically engineered proteins, including co-founding a start-up company (iGlyko, Inc.)
• Duties have included setting up laboratory space and assisting in creation of a research and business team through training and recruitment
• Scientific duties include work on fiber-optic system design, protein handling chemistries, hydrogel optimization, development of protocols, hardware automation, and detailed development planning
• Business duties include business plan writing/presentations, searching for funding from investors (individuals, investment bankers, and VCs), and webmaster

2007, FALL : RNA AMPLIFICATION ASSAY DEVELOPMENT
Gen-Probe, Inc., Dr. Norm Nelson
• Worked part-time in Gen-Probe’s biochemistry R&D group
• Developed and optimized various forms of Gen-Probe’s transcription-mediated amplification (TMA) assay for disease detection
• Reactions were run in a 96-well plate format using real-time molecular beacons for multiplexed detection
• Work included designing and carrying out experiments, as well as data analysis and quantitation
• Additional tasks involved instrumentation optimization such as protocol development for magnetic-bead based target-capture and automated well-plate dispensing

2001-2005 : RESEARCH ON MEMS NEURAL STIMULATION PROBES AND 3-D ELECTRODEPOSITION
UCLA, Dr. Jack Judy
• Worked in an EE MEMS lab on micro-machined, deep-brain stimulation probes for Parkinson’s Disease research
• Tasks included running tests to determine optimum electroplating conditions for micro-probe production, circuit design/construction, and packaging of external circuits
• Also worked on electroplating simulations involving 3-D modeling using Matlab, and validation of the models for accuracy against general, 3-D plated structures
• Was trained in UCLA's Nanofabrication facilities and well versed in the basic equipment and processes involving the fabrication of devices on Si wafers using oxides, nitrides, polysilicon, and metals in combination with deposition, masking, and etching

2004, SUMMER : QUANTITATIVE MICROSCOPY RESERACH
UCSD & The Burnham Institute, Dr. Jeff Price 
• Worked in various imaging labs, running phase-contrast image scans of tissue micro-arrays
• Was exposed to basics of phase-contrast, fluorescent, confocal, and automated microscopy
• Worked on image processing algorithms, specifically Matlab-based, edge-detection software
• Was exposed to basics of flow-cytometry

2002, SUMMER : BIOLISTICS RESEARCH
Lawrence Berkeley National Laboratory, Dr. Jian Jin   
• DOE/NSF sponsored undergraduate research fellowship program (ERULF) at the Lawrence Berkeley National Lab
• Research involved development of a biolistic gene gun for transfection of C. elegans roundworms
• Work included design modifications, experiment design, testing, a research paper, a poster session, and hands on skills such as metal machining, soldering, and programming

ACADEMIA EXPERIENCE

2008-CURRENT : IEEE JOURNAL OF MEMS REVIEWER
• Perform peer reviews of manuscripts submitted to the IEEE Journal of Microelectromechanical Systems

2007-2008 : STUDENT REPRESENTATIVE ON UC IRVINE, BIOMEDICAL ENGINEERING GRADUATE ADMISSIONS COMMITTEE
• Served with BME faculty to help review applications and decide on admission of graduate students
• Tasks included reading and sorting applications, researching unknown schools, initiating student contact, and helping make final admit decisions

PUBLICATIONS & CONFERENCES

2009 : JOURNAL PAPER, IN PREPARATION          TO BE SUBMITTED
R. Gorkin, J. Park, J. Siegrist, M. Amasia, B. Lee, J. Park, J. Kim, H. Kim, M. Madou, and Y. Cho, Centrifugal Microfluidics for Biomedical Applications, review manuscript

2009 : JOURNAL PAPER, UNDER REVIEW          SUBMITTED PUBLICATION
J. Siegrist, M. Amasia, N. Singh, D. Banerjee, and M. Madou, Numerical Modeling and Experimental Validation of Uniform Microchamber Filling in Centrifugal Microfluidics, manuscript

2009 : JOURNAL PAPER, UNDER REVIEW          SUBMITTED PUBLICATION
J. Siegrist and R. Gorkin, M. Bastien, G. Stewart, R. Peytavi, H. Kido, M. Bergeron, and M. Madou, Validation of a Centrifugal Microfluidic Sample Lysis and Homogenization Platform for Nucleic Acid Extraction with Clinical Samples, manuscript

2009 : JOURNAL PAPER, UNDER REVIEW          SUBMITTED PUBLICATION
J. Siegrist and R. Gorkin, L. Clime, E. Roy, R. Peytavi, H. Kido, M. Bergeron, T. Veres, and M. Madou, Serial Siphon Valving for Centrifugal Microfluidic Platforms, manuscript

2009-2010 : IVD TECHNOLOGY MAGAZINE, 3-ARTICLE SERIES
UPCOMING PUBLICATION
J. Siegrist, R. Peytavi, M. Bergeron, and M. Madou, Microfluidics for Biological Analysis: Triumphs and Hurdles of CD Platforms, Part 1 – Molecular Fundamentals (Nov./Dec.), Part 2 – Centrifugal Microfluidics (Jan./Feb.), Part 3 – A Vision for Tomorrow (Mar.)

2009 : MICROTAS CONFERENCE ABSTRACT & POSTER
Jeju, Korea (Nov.)          UPCOMING PUBLICATION
J. Siegrist and R. Gorkin, M. Bastien, G. Stewart, R. Peytavi, H. Kido, M. Bergeron, and M. Madou, Centrifugal Microfluidic Sample Lysis and Homogenization Platform for Nucleic Acid Extraction

2009 : OAK RIDGE CONFERENCE: FRONTIERS IN CLINICAL DIAGNOSTICS ABSTRACT & POSTER
Baltimore, MD (April)
N. Nelson, D. Lyakhov, S. Phelps, J. Chelliserrykattil, J. Carlson, M. Kaminsky, P. Gordon, S. Hashima, T. Ngo, J. Siegrist, and S. Brentano, Quantitative Detection of PCA3, PSA, and Internal Control in a Quantitative, Multiplex, Universal Real-time Transcription-Meditated Amplification Assay Format

2008 : MICROTAS CONFERENCE ABSTRACT & POSTER
San Diego, CA (Oct.)
E. Roy, J. Siegrist, R. Peytavi, G. Diaz-Quijada, H. Roberge, F. Normandin, G. Jia, J. Zoval, M. Madou, M. Bergeron, M. Dumoulin, and T. Veres, Thermoplastic Elastomers (TPE) Block Copolymers, A New Material Platform for Microfluidics: Proof-of-concept for Complex Siphon Valving on a CD

2008 : FRONTIERS IN BIOMEDICAL DEVICES CONFERENCE ABSTRACT & PRESENTATION
Irvine, CA (June)
J. Siegrist, M. Amasia, H. Kido, J. Zoval, and M. Madou, Microfluidic CD-Based Systems Toward Rapid Anthrax Detection in Whole Blood

2007 : SOCIETY OF EXPERIMENTAL MECHANICS CONFERENCE PAPER & PRESENTATION
Springfield, MA (June)
J. Siegrist, G. Jia, H. Kido, J. Zoval, G. Stewart, D. Gagné, R. Peytavi, A. Huletsky, M. Bergeron, and M. Madou, Centrifugal (CD) Microfluidic Platforms for Nucleic Acid Analysis

2007 : COLLOIDS AND SURFACES B JOURNAL PAPER
G. Jia, J. Siegrist, C. Deng, J. Zoval, G. Stewart, R. Peytavi, A. Huletsky, M. Bergeron, and M. Madou, (2007), A Low-Cost, Disposable Card for Rapid Polymerase Chain Reaction, Colloids Surf. B: Biointerfaces Special Edition, 58, 52-60.

2002 : LAWRENCE BERKELEY NATIONAL LAB RESEARCH SYMPOSIUM POSTER PRESENTATION
Berkeley, CA (Aug.)
J. Siegrist & J. Jin, Biolistic Gene Gun

PATENTS & DISCLOSURES

2009 : UC DISCLOSURE (July)       
J. Siegrist, R. Gorkin, R. Peytavi, and M. Madou, Centrifugal Microfluidic Sample Lysis and Homogenization Platform, UC Case No. 2010-036-1

2009 : UC DISCLOSURE (MARCH) 
J. Siegrist, M. Amasia, and M. Madou, Integrated, Centrifugal, Microfluidic System for Nucleic Acid Sample Preparation, Amplification, and Real-time Detection, UC Case No. 2009-544-1

2007 : UC DISCLOSURE (MAY)      
J. Siegrist, M. Madou, P. Wang, S. Daunert, E. Moschou, and S. Joel, Catheter for In Vivo Detection of Glucose, UC Case No. 2007-704-1; full patent application filed 2008

OTHER PUBLICATIONS & PRESS

2007 : NEWSPAPER ARTICLE (June)        
DNA tests get faster, smaller, cheaper, Orange County Register, Print & Online (June 28th)

AWARDS
• Graduate Dean’s Dissertation Fellowship : 2009
• Graduate Student Researcher Travel Award : 2007
• UC Irvine Biomedical Engineering Graduate Transition Fellowship : 2005

OTHER
• OCTANe Next Member : 2007-Current  
• BiON Cleanroom Facility Set Up : 2007-2008
-Worked as part of a team to establish and set up a biomedical-oriented cleanroom facility at UC Irvine
• BMES Member : 2005-Current 
• IEEE Member : 2002-Current