Experience

August 2002-present,
Technological coordinator: Infectious disease research center (IDRC) in Québec city, Canada. (Former Infectio Diagnostic Research bought by Beckton Dickison in 2006)
In charge of the scientific coordination between 7 multidisciplinary teams from private companies and academics, in order to design and develop a nucleic acid assay for rapid detection of infectious disease onto a Lab-on-Chip system. These teams are composed of molecular biologists, microbiologist, engineers, physicists, chemists, and physicians. This project is financed by a 10 million USD genome Canada grant.

1999-July 2002,
Post-Graduate researcher. Design and development of a Human Herpes Virus 8 (HHV8) microarrays.
Department of medicine, La Jolla CA, USA.
The focus of my work was to study and follow the expression of HHV8 genes in Kaposi’s sarcoma patients. For this purpose I developed a microarray containing 350 targets for the 88 orfs of HHV8 printed in aminosylane slides and set up a protocol suitable to label small RNA quantity (up to 1ug total RNA) with the dyes Cy3 and Cy5 without amplification. With this technique I was able to follow the expression of the viral genes directly from biopsies.

1995-1999,
Graduate student. Identification and characterization of a HIV-1 matrix cellular partner.
Institut de biologie, Universite de medecine, Montpellier France.

Using a two-hybrid system, I identified and characterized an interaction between HIV-1 matrix and the cellular protein HEED (Human Embryonic Ectoderm development).
I proposed a model where by interacting with the HIV-1 integration complex  this protein target the integration of viral genome in special areas of the cellular chromatin.

Skills and interests

Molecular biology
Microarray technology
Microfluidic
Molecular assays development
Coordination of multidisciplinary team
Biosensor (electrochemical, optical)
DNA computing and nanotechnology

Education

1999:   Ph.D in Molecular Biology.
Laboratoire de Virologie Moléculaire and Pathogénèse Virale, Pr Boulanger: “Identification and characterization of a cellular partner of the HIV-1 matrix protein”
University of medicine of Montpellier, France.

1992:   M.Sc. in Biochemistry
Paul Sabatier University, Toulouse, France.

1991:   Maitrise (B.Sc.) in Cellular Biology and Genetic.
Paul Sabatier University, Toulouse, France.

Languages

French: mother language
English: Fluent

Oral communication

«  Microfluidic Device for Rapid Automated Microarray Hybridisation » Advance Microarray Technology, London UK 2005

« Genomics and Microanalysis Systems » nanofabrication and biosensing, Boucherville, 2005

« Application in life science : Biosensing » Clean room inauguration, at Industrial Material institute at Boucherville, July 2004.

“Design and construction of a HHV8 microarray” Quebec, October 2001.

Publications/Papers

Picard FJ, Gagnon M, Bernier MR, Parham NJ, Bastien M, Boissinot M, Peytavi R, Bergeron MG. (2009) Internal control for nucleic acid testing based on the use of purified Bacillus atrophaeus subsp. globigii spores. J. Clin. Microbial (in press)

Zhengshan Zhao, Régis Peytavi, Gerardo A. Diaz-Quijada, Francois J. Picard, Ann Huletsky, Éric Leblanc, Johanne Frenette, Guy Boivin, Teodor Veres, Michel M. Dumoulin, and Michel G. Bergeron (2008) Plastic polymers for efficient DNA microarray hybridization: application to microbiological diagnostics. Journal of Clinical Microbiology, p. 3752-3758, Vol. 46, No. 11.

Karel Boissinot, Ann Huletsky, Régis Peytavi, Sophie Turcotte, Véronique Veillette, Maurice Boissinot, François J. Picard, Éric A. Martel, Michel G. Bergeron. (2007) Rapid exonuclease digestion of PCR-amplified targets for improved microarray hybridization. Clinical Chemistry  53(11):2020-3

Nicholas J. Parham, François J. Picard, Régis Peytavi, Martin Gagnon, Grégoire Seyrig, Pier-Ann Gagné, Maurice Boissinot and Michel G. Bergeron. (2007) Specific magnetic bead-based capture of genomic DNA from clinical samples: application to the detection of group B streptococci in vaginal/anal swabs. Clinical Chemistry. 53(9):1570-6.

Guangyao Jia, Jonathan Siegriest, Chengwu Deng, Jim V. Zoval, Gale Stewart, Régis Peytavi, Ann Huletsky, Michel G. Bergeron, Marc J. Madou. (2007) A low-cost, Disposable Card for Rapid Polymerase Chain Reaction. Colloids and surfaces B: Biointerfaces

Zhengshan Zhao, Gerardo A. Diaz-Quijada, Régis Peytavi, Éric Leblanc, Johanne Frenette, Guy Boivin, Jim V. Zoval, Marc Madou, Michel M. Dumoulin, Teodor, Veres, and Michel G. Bergeron. (2007) Detection of respiratory Viruses with plastic High Throughput Screening Devices. Mater. Res. Symp. Proc. Vol.950

Gerardo A. Diaz-Quijada, Regis Peytavi, André Nantel, Emmanuel Roy, Michel G. Bergeron, Michel M. Dumoulin, Teodor Veres. (2007) Surface modification of thermoplastics_towards low cost disposable high throughput screening Devices. Lab. Chip., 2007, 7, 856 – 862.

Guangyao Jia, Kuo-Sheng Ma, Jitae Kim, Jim V. Zoval, Régis Peytavi, Michel G. Bergeron and Marc J. Madou, (2006) Dynamic automated DNA hybridization on a CD (compact disc) fluidic platform, Sensors and Actuators B 114 173-181.

Peytavi R., Raymond F., Gagné D., Boissinot K., Picard F., Boissinot M., Bissonnette L., Ouellette M., and Bergeron M. (2005)  Microfluidic device for rapid (<15 min) automated microarray hybridization. Clinical Chemistry 51:10

Peytavi R., Tang L.,. Raymond F., Boissinot K., Bissonnette L., Boissinot M.,. Picard J, Huletsky A., Ouellette M.,.Bergeron M. (2005) Correlation between microarray DNA hybridization efficiency and the position of short capture probe on the target nucleic acid
BioTechniques 2005  Volume 39, Number 1: pp 89-96

Frédéric R Raymond* 1 , Hoang-Anh Ho* 2 , Régis Peytavi1 , Luc Bissonnette1 , Maurice Boissinot1 , François J Picard1 , Mario Leclerc2 and Michel G Bergeron1 (2005)Detection of target DNA using fluorescent cationic polymer and peptide
nucleic acid probes on solid support. BMC Biotechnology 2005, 5:10

Jia G.; Ma K.; Kim J.; Zoval J..; Madou M..; Deo. S.; Daunert. S.; Peytavi R; Bergeron M. (2004). CD (compact disc)-based DNA hybridization and detection MEMS, MOEMS, and Micromachining Volume: 5455.

Guimond C, Trudel N, Brochu C, Marquis N, El Fadili A, Peytavi R, Briand G, Richard D, Messier N, Papadopoulou B, Corbeil J, Bergeron MG, Légaré D, Ouellette M (2003). Modulation of gene expression in Leishmania drug resistant mutants as determined by targeted DNA microarrays. Nucleic Acids Res. 31(20): 5886–5896.
Marie-Catherine Romey, Nathalie Pallares-Ruiz, Alain Mange, Clément Mettling, Régis Peytavi, Jacques Demaille, and Mireille Claustres (2000) A Naturally Occurring Sequence Variation That Creates a YY1 Element Is Associated with Increased Cystic Fibrosis Transmembrane Conductance Regulator Gene Expression. J. Biol. Chem. 275: 3561-3567.
Peytavi R., Hong SS., Gay B., Dupuy d’Angeac A., Selig L., Bénichou S. Benarous R., Boulanger P. (1999). HEED, the product of the human homolog of the murine eed gene, binds to the matrix protein of HIV-1. J. Biol. Chem., 274, p1635-1645.
Hong SS., Galaup A., Peytavi R., Chazal N., Boulanger P., (1999). Enhancement of adenovirus (Ad)-mediated gene delivery by bimolecular oligopeptide with dual binding specificity. Hum Gene Ther. 1999 Nov 1 ; 10 : 2577-86
Bonnema A.B., Peytavi R., van Daelen R. A. J. Zabel P. Grimsley N., (1993) : Development of an vitro complementation system for identification of plants genes using yeast artificial chromosome (YAC). ACIIAR Prooceding, bacterial wilt ed Hartman GLP Hayward AC pp177-182.

Patents

Peytavi R, Martineau I, Belley-Montfort L, Huletsky A. Concentration and enrichment of microbial nucleic acids from blood specimens. Filled

Peytavi R, Kido H., Bergeron, G. M.(2007) Cascade siphon valves and their use in rotational microfluidic system. Provisional 60/ 904,372

Peytavi R, (2006) Electrochemical nucleic acid sensor mediated by enzymatic cascade. Provisional

• Peytavi R., Raymond F. (2005) Capture probes for efficient hybridization. CA2574917
• Peytavi R., (2005) Removable microfluidic system to drive reagents. EP1728062
• Bissonnette L., Peytavi R., Raymond F. (2004) System for charge-based detection of nucleic acids . EP1692309

Peytavi R., Veres A., Hogues H., (2006) Molecular Computer. CA2507187