Siegrist

Jonathan P. Siegrist

OBJECTIVE

Obtain a Ph.D. degree in engineering with an emphasis in biomedical devices. The idea of applying miniaturization technologies to the fields of biology and medicine greatly appeals to me, and so I would prefer to work on bioMEMS-related research projects. My future career plans involve being at the head of biomedical research projects in industry, as either part of a start-up or established company. I would eventually like to incorporate part-time community-college teaching into my career as well.

EDUCATION

2000High School Diploma

Las Lomas High School-Walnut Creek, CA

2005B.S., Electrical Engineering with Biomedical Engineering Option

University of California, Los Angeles

Cumulative UCLA GPA: 3.42

2006M.S., Biomedical Engineering

University of California, Irvine

Cumulative UCI GPA: 4.00

2006-Present Ph.D., Biomedical Engineering

University of California, Irvine

Advisor: Dr. Marc Madou

Current Cumulative UCI GPA: 3.99 (Advancement to Candidacy: 8/23/07)

Life Sciences Coursework:

Evolution, Ecology Cells, Tissues, and OrgansMolecular Biology

GeneticsOrganic Chemistry and Lab Tissue Engineering

Quantitative PhysiologyBiochemistry

Clinical Medicine (including in-room observations of surgeries at UCI Medical Center)

Engineering Coursework:

Systems and Signals (Digital and Analog)Feedback ControlDigital Logic Design

Circuit Design and Design LabsThermodynamicsFluid Mechanics

Electromagnetics and WavesSpeech and Image ProcessingLasers and Quantum ElectronicsMEMS with Fabrication Lab Micro-Bio Implants BioMEMS

Micro-Bio Analysis Fluorescence Spectroscopy

Programming/Software Skills:

Microsoft OfficeMatlabPSpice

C++L-EditWindows and Mac OS X

LabVIEWSolidWorksAdobe Illustrator

AutoCAD

Manufacturing/Microfabrication Skills:

CNC MachiningMetal and Plastics MachiningE-Beam Deposition

PhotolithographySputteringRIE/DRIE

Electrodeposition/polymerizationProfilometrySoft Lithography

RESEARCH EXPERIENCE

2006-CurrentCD-Based Microfluidic Diagnostics & Glucose Sensor Research, Dr. Marc Madou, UCI

•Constructing microfluidic PCR & DNA hybridization platforms to integrate onto a CD-based total analysis system for rapid, automated infectious disease detection as part of a Genome Canada-Génome Québec initiative

•Also developing a CD-based system for anthrax detection that combines cell lysis with qPCR

•Using CNC machining, hot-embossing, and soft lithography to manufacture disposable microfluidic devices

•Automating CD platforms for fluorescence detection and developing a feedback control system for PCR thermocycling

•Working in UCI cleanroom facilities on microfabrication of devices

•Also working on implantable, fiber-optic glucose sensor based on genetically engineered proteins, including co-founding a start-up company (iGlyko) and looking for seed-stage funding from investors

Fall 2007RNA Amplification Assay Development, Dr. Norm Nelson, Gen-Probe, Inc.

•Worked part-time in Gen-Probe’s biochemistry R&D group

•Developed and optimized various forms of Gen-Probe’s transcription-mediated amplification (TMA) assay for disease detection

•Reactions were run in a 96-well plate format using real-time molecular beacons for multiplexing

•Work included designing and carrying out experiments, as well as data analysis and quantitation

•Work also included instrumentation optimization, such as protocol development for magnetic-bead based target-capture using KingFisher instruments

2001-2005MEMS Neural Stimulation Probes and 3-D Electrodeposition Research, Dr. Jack Judy, UCLA

•Worked in an EE MEMS lab on micro-machined, deep-brain stimulation probes for Parkinson’s Disease research

•Tasks included running tests to determine optimum electroplating conditions for probe production, circuit design/construction, and packaging of external circuits

•Also worked on electroplating simulation involving 3-D modeling using Matlab, and testing of these models for accuracy against general, 3-D plated structures

•Was trained in UCLA's Nanofabrication facilities and well versed in the basic equipment and processes involving the fabrication of devices on Si wafers using oxides, nitrides, polysilicon, and metals in combination with deposition, masking, and etching

Summer 2004Quantitative Microscopy Research, Dr. Jeff Price, UCSD and The Burnham Institute

•Worked in imaging labs, running phase-contrast image scans of tissue micro-arrays

•Was exposed to basics of phase contrast, fluorescent, confocal, and automated microscopy

•Worked on image processing aspects, specifically Matlab-based, edge-detection software

•Was exposed to basics of flow-cytometry

Summer 2002Biolistics Research, Dr. Jian Jin, Lawrence Berkeley National Laboratory

•DOE/NSF sponsored undergraduate research fellowship program (ERULF) at the Lawrence Berkeley National Lab

•Research involved development of a biolistic gene gun for transfection of C. elegans roundworms

•Work included design modifications, experiment design, testing, a research paper, a poster session, and hands on skills such as metal machining, soldering, and programming

ACADEMIA EXPERIENCE

2008-CurrentIEEE Journal of MEMS Reviewer

•Perform peer reviews of manuscripts submitted to the IEEE Journal of Microelectromechanical Systems

2007-2008Student Representative on UC Irvine, Biomedical Engineering Graduate Admissions Committee

•Served with BME faculty to help review applications and decide on admission of graduate students

•Tasks included reading and sorting applications, researching unknown schools, initiating student contact, and helping make final admit decisions

PUBLICATIONS/CONFERENCES/PRESENTATIONS

2008 uTAS Conference Abstract & Poster-San Diego, CA (October, 2008)

•“Thermoplastic elastomers (TPE) block copolymers, a new material platform for microfluidics: Proof-of-concept for complex siphon valving on CD.” E. Roy, J. Siegrist, R. Peytavi, G. A. Diaz-Quijada, H. Roberge, F. Normandin, G. Jia, J. Zoval, M. Madou, M. G. Bergeron, M.M. Dumoulin, T. Veres.

2008 DARPA All-Center Conference Poster-Minneapolis, MN (June, 2008)

•Experimental Validation of Numerical Models for Centrifugal Microfluidics used for Anthrax Detection

2008 Frontiers in Biomedical Devices Conference Abstract & Presentation-Irvine, CA (June, 2008)

•Microfluidic CD-Based Systems Toward Rapid Anthrax Detection in Whole Blood

2008 DARPA-MF3 Center Conference Poster-Miami, FL (January, 2008)

•Microfluidic CD-based system for rapid anthrax detection in whole blood using real-time PCR

2007 Nanotech Conference Abstract & Poster-Montreux, Switzerland (November, 2007)

•“Thermoplastic elastomeric (TPE) block copolymers, a new material platform for microfluidics: Proof-of-concept for complex siphon valving on a CD.” E. Roy, J. Siegrist, R. Peytavi, G. A. Diaz-Quijada, H. Roberge, F. Normandin, G. Jia, J. Zoval, M. Madou, M. G. Bergeron, M.M. Dumoulin, T. Veres.

2007 DARPA-MF3 Center Conference Poster Presentation-Berkeley, CA (June, 2007)

•Long-Term, Continuous Glucose Sensing using Genetically Engineered Proteins

2007 Society of Experimental Mechanics Conference Paper & Presentation–Springfield, MA (June, 2007)

•Jonathan Siegrist, Guangyao Jia, Horacio Kido, Jim Zoval, Gale Stewart, Dominic Gagné, Régis Peytavi, Ann Huletsky, Michel Bergeron, Marc Madou, Centrifugal (CD) Microfluidic Platforms for Nucleic Acid Analysis, SEM Conference, (2007).

2007 Journal Paper (March, 2007)

•Guangyao Jia, Jonathan Siegrist, Chengwu Deng, Jim V. Zoval, Gale Stewart, Régis Peytavi, Ann Huletsky, Michel G. Bergeron, Marc J. Madou, (2007). A Low-Cost, Disposable Card for Rapid Polymerase Chain Reaction, Colloids Surf. B: Biointerfaces Special Edition, 58(1), 52-60. Click here for PDF.

2007 DARPA-MF3 Center Conference Poster Presentation-Irvine, CA (January, 2007)

•Rapid, Disposable-Card Based Polymerase Chain Reaction System

2002Abstract Published in NSF Undergraduate Research Journal (September, 2002)

•Biolistic Gene Gun

Summer 2002Lawrence Berkeley National Laboratory Research Symposium Poster Presentation-LBNL (August, 2002)

•Biolistic Gene Gun

PATENTS/DISCLOSURES

2007UC Disclosure – Catheter for in vivo detection of glucose (May, 2007)

•Jonathan Siegrist, Marc Madou, Ping Wang, Sylvia Daunert, Elissabeth Moschou, Smita Joel

•UC Case No. 2007-704-1

•Full patent filed August 2008

OTHER PUBLICATIONS/PRESS